Form 4 · Worked answers

Movement of Substances Across a Plasma Membrane, worked answers

Fully worked answers for Movement of Substances Across a Plasma Membrane, original structured and essay questions with mark-scheme keywords highlighted.

How this topic is examined

  • Defining osmosis precisely and predicting whether a cell gains or loses water.
  • Explaining visking tubing or potato-strip experiment results.
  • Comparing diffusion, osmosis and active transport in a table.
  • Explaining, using water potential or concentration language, why water moves into or out of a cell placed in a stated solution, and stating the direction and the reason together for full marks.
  • Interpreting a graph or data table that shows how surface area, temperature or concentration affects the rate of diffusion, osmosis or active transport, and identifying which variable was changed.
  • Applying active transport correctly to mineral-ion or glucose absorption against a concentration gradient, linking it to energy from respiration and to carrier proteins rather than channel proteins.
  • Recognising that osmosis is a special case of diffusion that applies only to water molecules moving across a partially permeable membrane, rather than treating the two terms as interchangeable.

Model answer structure

  1. Read the command word and answer to the marks, one clear point per mark.
  2. Define the key biological term precisely before you explain it.
  3. Explain the process or reason in the correct sequence, using the right terms.
  4. Where useful, add a labelled diagram or a worked example.
  5. End with the link the question asks for (cause → effect, structure → function).

Fully worked answers

1

A student places a red blood cell in a beaker of 5% salt solution, which is hypertonic to the cell's cytoplasm. State and explain what happens to the red blood cell.

[4]
Show answer

The solution has a lower water potential than the cytoplasm inside the red blood cell, so water moves out of the cell by osmosis, down the water potential gradient, across the partially permeable plasma membrane. As the red blood cell loses water it shrinks and its surface becomes crinkled, a change called crenation, because the cell has no cell wall to resist this loss of water.

lower water potentialosmosiswater potential gradientpartially permeable membranecrenation

2

Visking tubing containing a starch solution is tied and immersed in a beaker of iodine solution. After 30 minutes, the tubing turns blue-black but the iodine solution outside remains orange-brown. Explain this observation.

[5]
Show answer

Visking tubing is partially permeable, with pores small enough to let small molecules such as iodine pass through but not large starch molecules. Iodine molecules diffuse from a region of higher concentration outside the tubing to a region of lower concentration inside, down the concentration gradient, and react with the starch to produce a blue-black colour inside the tubing. Starch molecules are too large to diffuse out through the pores of the tubing, so the iodine solution outside stays orange-brown because no starch reaches it to react.

partially permeablediffusionconcentration gradientstarch too largeblue-black colour

3

Compare diffusion and active transport in terms of the direction of movement, the need for energy, and one example of each in the human body.

[6]
Show answer

Diffusion moves particles down the concentration gradient, from a region of higher to lower concentration, and needs no energy because it happens through the random kinetic movement of particles; an example is the diffusion of oxygen from the alveolus into the surrounding blood capillary. Active transport moves particles against the concentration gradient, from a region of lower to higher concentration, and requires energy from respiration in the form of ATP together with a specific carrier protein; an example is the reabsorption of glucose from the filtrate back into the blood in the kidney tubule, even when the blood glucose concentration is already higher than that in the filtrate.

down the gradientno energyagainst the gradientATPcarrier proteinkidney tubule reabsorption

4

Root hair cells absorb mineral ions such as nitrate from the soil even though the soil solution has a lower ion concentration than the cell sap. Explain how this is possible and name the process involved.

[5]
Show answer

Because the mineral-ion concentration in the soil solution is lower than inside the root hair cell, the ions cannot move in by diffusion, which only works down a concentration gradient. Instead, the root hair cell uses active transport: energy released from respiration in the mitochondria is used by specific carrier proteins in the plasma membrane to pump mineral ions into the cell against their concentration gradient. This is why root hair cells contain many mitochondria, to supply the ATP that active transport of mineral ions requires.

against concentration gradientactive transportrespirationATPcarrier proteinmitochondria

5

An investigation measures the rate of diffusion of a dye through agar blocks of different sizes but the same shape. Explain why the smallest block reaches complete colour change fastest, using the idea of surface area to volume ratio.

[5]
Show answer

A smaller cube has a larger surface area to volume ratio than a larger cube of the same shape, so relative to its volume, the smallest block has more surface exposed for the dye to diffuse in across. Because the diffusion distance from the surface to the centre of the block is also shorter in the smallest cube, the dye reaches the centre faster, so the smallest block shows complete colour change first even though diffusion happens at the same rate per unit area across all the blocks.

surface area to volume ratiosmaller blockshorter diffusion distancesame rate per unit area

6

Explain why raising the temperature from 25°C to 60°C increases the rate of diffusion of a dye in water but decreases the rate of active transport of glucose into a cell.

[6]
Show answer

Raising the temperature gives particles more kinetic energy, so the dye molecules move and collide faster, increasing the rate of diffusion, and this effect on diffusion continues to rise as temperature rises within a normal range. Active transport, however, depends on carrier proteins in the plasma membrane, and proteins are made of amino acid chains held in a specific three-dimensional shape by bonds that break down at high temperature; at 60°C the carrier proteins denature and lose their functional shape, so they can no longer bind and transport glucose, which lowers the rate of active transport even though the temperature is higher.

kinetic energyrate of diffusion increasescarrier proteindenatureactive transport decreases

Phrasing that earns marks

  • Fluid mosaic model: A phospholipid bilayer with proteins scattered through it; it is partially (selectively) permeable.
  • Diffusion: Net movement of particles from high to low concentration, down a concentration gradient, without energy.
  • Osmosis: Net movement of water molecules from a less concentrated (dilute) to a more concentrated solution across a partially permeable membrane.
  • Active transport: Movement of substances against the concentration gradient, using energy from respiration and carrier proteins.
  • Effects on cells: In hypotonic solution animal cells burst (haemolysis) and plant cells become turgid; in hypertonic solution animal cells shrink (crenation) and plant cells plasmolyse.
  • Applications: Osmosis and diffusion explain wilting, food preservation by salting, and root absorption of minerals by active transport.
  • Membrane transport proteins: Channel proteins form a fixed, water-filled pore that lets specific ions or small polar molecules diffuse straight through the membrane. Carrier proteins instead bind the particle, change shape and release it on the other side; they can work passively in facilitated diffusion or, using ATP, actively pump a substance against its concentration gradient.
  • Factors affecting rate of movement: The rate of diffusion, osmosis and active transport increases with a steeper concentration gradient, a larger surface area, a smaller particle size and a higher temperature, because particles then have more kinetic energy and collide with the membrane more often; active transport is further limited by the amount of ATP and carrier proteins available.

Frequently asked questions

What is osmosis in simple terms?
Osmosis is the net movement of water molecules across a partially permeable membrane, from a solution with more water (less concentrated) to one with less water (more concentrated). No energy is needed, so it is a form of passive transport.
How is active transport different from diffusion?
Diffusion moves particles down the concentration gradient with no energy. Active transport moves them against the gradient and needs energy from respiration plus carrier proteins, which is how roots absorb mineral ions even when the soil has a lower concentration.
Why does a plant wilt when the soil is dry or too salty?
When the surrounding solution is more concentrated than the cell sap, water leaves the cells by osmosis. The cells lose turgor and become flaccid, and if it continues the cell membrane pulls away from the wall (plasmolysis), so the plant wilts.

More for Movement of Substances Across a Plasma Membrane

Related

Book a Trial ClassOne-hour paid trial · Same-day reply